Otto Folin contributed to clinical chemistry by introducing Jaffe's method for creatinine measurement. Which analyte did this method measure?

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Multiple Choice

Otto Folin contributed to clinical chemistry by introducing Jaffe's method for creatinine measurement. Which analyte did this method measure?

Jaffe's method is a classic colorimetric assay for creatinine. In alkaline solution, picric acid reacts with creatinine to form a red-orange creatinine-picrate complex, and the color intensity is measured to determine creatinine concentration. Creatinine is produced at a relatively constant rate and is filtered by the kidneys, so its level reflects renal function, which is why Folin helped popularize this method. Among the options, the analyte measured by this method is creatinine. Epinephrine, uric acid, and protein require different assays and are not detected by the Jaffe reaction.

Jaffe's method is a classic colorimetric assay for creatinine. In alkaline solution, picric acid reacts with creatinine to form a red-orange creatinine-picrate complex, and the color intensity is measured to determine creatinine concentration. Creatinine is produced at a relatively constant rate and is filtered by the kidneys, so its level reflects renal function, which is why Folin helped popularize this method. Among the options, the analyte measured by this method is creatinine. Epinephrine, uric acid, and protein require different assays and are not detected by the Jaffe reaction.

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